This is recognized as among the highest longevity rates anywhere – and the islanders have a tendency to live out their much longer lives in good health. Gerasimos Siasos, a medical professor and doctor at the University of Athens Medical School, Greece set out along with his team to find out whether the elderly population's coffee drinking got an effect on their health. In particular, the experts investigated links between coffee-drinking habits and the subjects' endothelial function. The endothelium is usually a level of cells that lines arteries, which is normally affected both by ageing and by way of life habits .Isolation of Factor IX from Plasma and Sequencing of the Factor IX Gene From 40-ml samples of plasma obtained from the proband and from the controls, factor IX was isolated through affinity chromatography with the use of antihuman factor IX Sepharose and through ion-exchange chromatography with the use of Q Sepharose Fast Flow moderate.9 Genomic DNA was acquired, and the factor IX gene was sequenced with the use of the ABI PRISM 3700 DNA Sequence Detection Program . Recombinant Factor IX Human embryonic kidney 293 cells were transduced by lentivirus vectors that expressed wild-type aspect IX or aspect IX with the substitution of leucine for arginine at position 338 by using the ViraPower HiPerform Expression System . The aspect IX concentration was determined by using an enzyme-connected immunosorbent assay.10 Functional factor IX activity was measured by means of a clotting assay with the use of a modified one-stage factor assay.5 percent affinity-purified goat antihuman factor IX .